Movement of M1 Molecules in Living CHO Cells
This video shows the movement of M1 muscarinic acetylcholine receptor molecules labelled with florescent ligand Cy3B--telenzepine in living CHO cells using TIRFM (total internal reflection fluorescence microscopy) and Andor iXon EMCCD camera.
The M1 molecules undergo unrestricted pseudo 1-D diffusion on filopodia membrane and 2-D diffusion on plasma membrane. Muscarinic acetylcholine receptors are the members of the superfamily of GPCR.
Run Time – 0:06sec