Photoactivation in Discrete Regions of GAG-GFP for Particle Tracking
This video from Paul Spearman MD., at Emory shows the simultaneous photoactivation of the key HIV structural proteins (PA/Gag/GFP) for particle tracking in the nucleus and cell membrane boundary.
Photoactivation is performed in order to study the movement of these protein molecules. The images were captured in real time using Andor’s Mosaic platform and laser spinning disc confocal microscopy.
Run Time – 0:13sec