Diffraction Limited Photoactivation of GFP with TIRF
This Mosaic video by Andor Technology shows the photoactivation of GFP (green fluorescent protein) in multiple regions of interests at the diffraction limit.
The images were acquired using a real-time Nikon TIRF (total internal reflection fluorescence) system. The Mosaic is capable of simultaneous illumination and imaging.
Run Time – 0:20sec