MKS Inc., a global provider of enabling technologies that transform our world, has announced the next generation of Newport™ ODiate® Fluorescence Filter Sets, an expanded filter portfolio that includes precision single-band optical filters as well as new multi-band optical filters designed for fluorescent imaging.
The new DAPI/TRITC and FITC/Cy5 dual-band filters provide single-band filter level signal performance in multi-band filters, allowing for the simultaneous detection of multiple fluorescent tags in a single sample. This means when pairing alternating fluorophores, high throughput passbands can be designed to deliver higher signal strength than can be expected with adjacent fluorophores.
With just two measurements, the DAPI/TRITC and FITC/Cy5 sets can be used together to achieve 4-channel multiplex imaging with fluorescence results similar to single-band imaging filter sets. The new multi-band filter sets offer advantages in fluorescence microscopy, flow cytometry, and PCR applications.
Image Credit: MKS Inc.
"Imaging OEMs and researchers are looking for options that produce bright, high-contrast images at an affordable price," said Dr. Glenn Mitchell, General Manager, Newport Components and Vibration Control. "The new ODiate multi-band fluorescence filter sets deliver exactly that. They simultaneously image multiple fluorophores in a single sample, enabling rapid, multi-color imaging. The entire ODiate family of single-band and multi-band filter sets is another example of MKS's commitment to advancements in the Life and Health Science field."
Fluorescence - a widely used technique in biology, biotechnology, and medicine, due to its extraordinary sensitivity, high specificity, and ease of use - occurs when a molecule absorbs light at wavelengths within its absorption band and then emits light at longer wavelengths within its emission band.
Newport ODiate Fluorescence Filter Sets, which include emitter, exciter and dichroic filters, are optically designed to deliver optimal imaging responses from some of the most common fluorophores used in fluorescent imaging systems. The filter sets feature high % transmission, steep spectral edges, and deep OD, enabling high image quality and low signal to noise ratio in fluorescent imaging systems.
Newport ODiate Fluorescence Filter Sets utilize proprietary thin film coating technology to provide precision, durable optical filters. The filters are fabricated at industry-standard dimensions that make them compatible with filter cubes from all major manufacturers. Individual single-band filters as well as filter sets are targeted at common fluorophores: DAPI, TRITC, GFP, FITC, Texas Red, and Cyanine (CY3.5).
The new dual-band sets are offered for DAPI / TRITC and FITC / Cy5. All ODiate filter sets are designed to produce bright, high-contrast images and to maximize signal-to-noise ratios. The filter sets are ideal for fluorescence microscopy or fluorescence imaging applications. Additionally, they can be utilized in polymerase chain reaction (PCR) diagnostic instruments.
Customization Options
MKS is recognized as a leader for precision optical systems for highly functioning optical systems. MKS also works with customers at the application and system level to design and manufacture custom-designed optical filter systems that meet or exceed system requirements, following fluorophore usage trends, light source innovations, and detector enhancements. The company can supply optical filters at the individual component level or as part of an integrated optical sub-assembly.
Availability
The expanded Newport ODiate Fluorescent Filter Sets are available now.