Onefive GmbH continues the efforts into the development of pulsed ps laser sources for microscopy and several other applications. After customizing the Katana-08 HP as depletion STED laser for super-resolution microscopy, for which the company signed an exclusive supplier agreement with Leica Microsystems, Onefive releases a new series of single-box multi-color ps laser systems.
Given the increasing demand for multi-wavelength excitation lasers for microscopy, the Katana HP fiber laser sources now come together in a single packaging solution to provide multi-color laser sources. The first dual-color excitation system, at 532 nm and 635 nm, has been already tested and integrated with the STED depletion laser at 775 nm to offer Leica Microsystems a complete laser solution for STED microscopy.
Combining the 532/635 nm excitation colors with the 775 nm depletion laser proved to give very efficient results for several dyes. Other combinations of excitation and depletion wavelengths for many other dyes are also possible.Upon customer request Onefive can provide different multiple-color excitation and depletion laser sources, as reported in the table below.
| Excitation Wavelength (nm) |
Depletion Wavelength (nm) |
| 532 |
775 |
| 595 |
775 |
| 635 |
775 |
| 640 |
775 |
| 488 |
647 |
| 470 |
647 |
| 570 |
690 |
Example of possible combination of excitation/depletion wavelengths that Onefive can provide for multicolor STED microscopy.
The Katana platform offers the versatility of continuously tuning the pulse repetition rate, from pulse on demand up to 120 MHz. Pulse duration can range from sub 20 ps up to 1 ns. Various wavelengths ranging from the near infrared, the visible to the UV are available. Onefive lasers are designed and qualified for 24/7 operation under harsh environments. A focused product development approach provides a dust-sealed, maintenance-free small footprint product with reliable turn-key operation at a competitive price throughout the complete product fleet.