The new upgrade kit expands the capabilities of the laser scanning microscope Olympus FluoView FV3000 with time-resolved analysis methods.
PicoQuant GmbH has expanded the compatibility list of its FLIM and FCS upgrade kit to include the Olympus FV3000 confocal laser scanning microscope (CLSM). The upgrade kit is an external addition to the CLSM enhancing its capabilities by the addition of time-resolved techniques such as fluorescence lifetime imaging (FLIM), Fluorescence Correlation Spectroscopy (FCS), or advanced time-resolved Förster Resonance Energy Transfer (FRET) analysis. Even the new approach rapid2FLIM for very fast and accurate FLIM imaging is available.
The turn-key system consists of three major parts: picosecond pulsed excitation sources, single photon sensitive detection systems, and Time-Correlated Single Photon Counting (TCSPC) data acquisition hardware. The internal cw and picosecond pulsed lasers are coupled into the scan head via a polarizing beamsplitter that allow using cw and pulsed excitation simultaneously. The broad range of laser sources available (from 405 nm to 640 nm), high photon sensitivity, and time resolutions ranging from pico- to microseconds make it possible to analyze all types of commonly used fluorophores and samples.
PicoQuant's FLIM and FCS upgrade kit is a complete set of instruments that can be adapted to Olympus LSMs in various configurations. The compact and easy-to-use system extends the capabilities of the LSM towards time-resolved measurements with resolutions down to picoseconds and allows performing various analyses on this time scale.