Olympus has unveiled the new XLPLN25XSVMP SCALEVIEW 25x objective lens (NA 1.0) for an intensified imaging of condensed biological samples.
In association with the RIKEN Brain Institute of Japan, the innovative SCALEVIEW approach was established, which facilitates researchers to generate precise 3D structural image of brain tissue. In order to counter limitations like inadequate light scattering and tissue opacity effects, the new innovation has an extensive distance of 4 mm, concurrent to the SCALEVEW-A2 clearing agent by Olympus, resulting in clear samples, thereby maintaining fluorescent signals. The new objective functions with Olympus’ FluoView FV1000MPE multiphoton system to facilitate deep penetration into the samples related to intact specimens.
In order to improve multiphoton microscopy operation, the SCALEVIEW-A2 reagent and SCALEVIEW 25x objective are designed, with fluorescent markers creating accurate reconstructions up to a depth of 4mm. By clarifying the tissues with formalin and illuminating the sample, the SCALEVIEW-A2 reagent facilitates a specific data creation without sectioning that exhibits the internal structure of a complex specimen accurately. This technique supports the development of 3D representations.
In addition to the benefits of SCALEVIEW-A2 clearing solution, Olympus has launched the SCALEVIEW 25x objective, for powerful imaging. The multiphoton objective with extensive capacity facilitates the high-precision imaging of translucent biological specimens up to 4mm depth. The objective supported with a correction collar is optimized for functioning with the SCALEVIEW-A2 reagent (refractive index 1.38), thereby producing specific images. The new SCALEVIEW technology incorporates with Olympus’s FluoView FV1000MPE multiphoton microscopes constantly, as a part of a complete system. The FV10-ASW software v3.1, generates the energy to visualize 3D structures in morphologically intact tissues, at unusual depths.
Source: http://www.microscopy.olympus.eu