In this latest study, the researchers collected samples of carious dentin from three patients and formed biofilms in the laboratory. The microbial composition of the biofilms varied among the patients, comprising streptococci, lactobacilli, and yeasts.
The biofilms were treated with PDT associated with LED irradiation mediated by a photosensitizer derived from chlorin e-6 (Fotoenticine), and then analyzed by counting the colony-forming units (CFU) in selective and non-selective culture media.
Overall, PDT eliminated S. mutans and decreased total microorganisms by up to 3.7 units (log10 CFU). In the case of streptococci, the reduction was 2.8, and for lactobacilli and yeasts it was 3.2 each. The reductions indicated approximately 99% efficacy of antimicrobial activity.
The treatment was also shown to disaggregate the biofilms and reduce acid concentration (which contributes to caries formation) by 1.1 to 1.9 mmol lactate/L. Biofilm structure and acid production by microorganisms were analyzed using microscopic and spectrophotometric analysis respectively.
Dental caries are influenced by lifestyle, diet, oral hygiene, and the presence of fluoride in the water supply or toothpaste. Genetic factors may also play a role.
"Dentistry has advanced significantly in recent years, but caries still need to be treated using rotary instruments and drills, which can cause discomfort and anxiety in patients," Junqueira told Agência FAPESP. "PDT is an auxiliary treatment. In children with extensive carious lesions, for example, drilling can lead to loss of structure in non-affected teeth as well as patient distress. An alternative is manual removal of caries and use of PDT with chlorin e-6. This is quick and painless while preserving dental structure."
PDT is a chemical process resulting from the interaction of light, oxygen, and a drug sensitive to light – a photosensitizer – that is administered in the form of an ointment or injected. After a certain interval, the tissue is irradiated with light at a specific wavelength depending on the drug. The light activates the drug, which reacts with the oxygen in the infected cells, resulting in their death.
Treatments for caries include application of fluoride, fillings, and placement of crowns. Severe cases may require root canal treatment or tooth extraction. PDT is already used by dentists, she added, but the most widely used photosensitizer is methylene blue, which is a dye and must be used sparingly to avoid staining the teeth.
"Our search for other photosensitizers led us to chlorin e-6 because of its satisfactory results. The advantages include the fact that it isn't a dye and doesn't stain the teeth, as well as the use of light at wavelengths in the range of 650-660 nanometers, compatible with the equipment dentists already have in their consulting rooms," Junqueira said.
"Many applications of PDT have been researched, against skin infections and cancer, for example, as well as leishmaniasis, but its use against microorganisms is also being studied, as is its application to treat oral cavity lesions, which led to our decision to pursue this avenue."
Another innovative aspect of the study, according to Junqueira, was the use of microcosm biofilm developed from samples donated by three patients at dental clinics in São José dos Campos aged 20-40 and with at least one dentin caries lesion in a molar tooth. Microcosm biofilm is an in vitro version of natural oral biofilm.
The human oral cavity can contain as many as 700 microbial species, of which at least 40 have been associated with caries. "It's hard to reproduce in vitro the microbial environment found in saliva and oral biofilm. We succeeded in doing so," she said.
Little is known about the oral health of Brazilians in general. A project called SB Brazil is conducted under the aegis of the National Oral Health Policy and expected to publish statistics later this year.
The last nationwide survey, performed by the Ministry of Health in 2010, found that 46.6% of under-fives had no deciduous (milk) teeth caries, while 43.5% of 12-year-olds had healthy permanent teeth. The proportions were 23.9%, 0.9% and 0.2% in the 15-19, 35-44 and 65-74 age groups respectively.