Posted in | News | Imaging

BLAZE® Enables Ultra-Multiplex CARS Spectroscopic Imaging



Teledyne Princeton Instruments, a world-renowned manufacturer of scientific imaging and spectroscopy equipment, and a Teledyne Technologies [NYSE:TDY] company, is pleased to recognize the recent work of Associate Professor Hideaki Kano of the University of Tsukuba, Japan. Earlier this year, Dr. Kano and his colleagues performed ultra-multiplex CARS spectroscopic imaging (18 colors corresponding to 18 wavenumbers; 600 cm-1 – 3600 cm-1) on live A549 cells, reporting an effective exposure time per pixel of 1.8 msec.†

Coherent anti-Stokes Raman scattering, or CARS, allows unlabeled molecules in the human body to be identified via spectral analysis. Ultra-multiplex CARS is broad enough to detect all vibrational fundamental modes, including the critically important fingerprint region (in which unknown organic compounds can be identified and distinguished from one another) as well as the C-H and O-H stretching regions (useful for identifying the rough molecular distribution of lipids, proteins, and nucleic acids). Dr. Kano explains that the combination of this real-time, non-invasive, label-free technique and multivariate analysis methods can help life scientists and medical doctors gain meaningful insights into the dynamics of intracellular metabolic activity.

For their experiments, Dr. Kano’s research group chose a new Teledyne Princeton Instruments BLAZE camera with a proprietary ‘super-deep-depletion’ CCD designed to yield the highest near-infrared quantum efficiency of any silicon device available. The new sensor platform’s dual 16 MHz readout ports enable unprecedented spectral rates of more than 1600 spectra/second with full vertical binning and up to 215 kHz when operated in kinetics mode. The camera features a back-illuminated 1340 x 400 spectroscopic array format (20 μm square pixels) capable of being cooled down to -95°C in air, without chillers or liquid assist, for low-dark-current performance. A Teledyne Princeton Instruments LS-785 spectrograph was also utilized, as was Teledyne Princeton Instruments LightField® software.

Before I used BLAZE, I thought the possibilities of CCD fabrication technology were actually becoming somewhat saturated. However, BLAZE has drastically changed my impression. This camera signals a bright future for spectroscopy!

Dr. Kano, University of Tsukuba

Ultra-multiplex CARS and Dr. Kano’s recent work are discussed in a new application note from Teledyne Princeton Instruments, “Ultra-Multiplex CARS Spectroscopic Imaging of Living Cells”. Currently, Dr. Kano’s research group is collaborating with a pathologist to develop a new spectroscopic diagnostic tool using the molecular fingerprint.

† Hideaki Kano, Takumi Maruyama, Junko Kano, Yuki Oka, Daiki Kaneta, Tiffany Guerenne, Philippe Leproux, Vincent Couderc, and Masayuki Noguchi, “Ultra-multiplex CARS spectroscopic imaging with 1-millisecond pixel dwell time,” OSA Continuum 2, 1693–1705 (2019). Open access:


Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Teledyne Princeton Instruments. (2019, December 06). BLAZE® Enables Ultra-Multiplex CARS Spectroscopic Imaging. AZoOptics. Retrieved on July 12, 2024 from

  • MLA

    Teledyne Princeton Instruments. "BLAZE® Enables Ultra-Multiplex CARS Spectroscopic Imaging". AZoOptics. 12 July 2024. <>.

  • Chicago

    Teledyne Princeton Instruments. "BLAZE® Enables Ultra-Multiplex CARS Spectroscopic Imaging". AZoOptics. (accessed July 12, 2024).

  • Harvard

    Teledyne Princeton Instruments. 2019. BLAZE® Enables Ultra-Multiplex CARS Spectroscopic Imaging. AZoOptics, viewed 12 July 2024,

Tell Us What You Think

Do you have a review, update or anything you would like to add to this news story?

Leave your feedback
Your comment type

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.