Optical cubes are considered the quiet directors of fluorescence systems, leading excitation and emission light through a formal arrangement of filters and mirrors. Following an efficient assembly procedure allows users to achieve accurate spectral performance, a great signal-to-noise ratio, and dependable imaging results.
Filter cubes are generally made up of three core elements:
- Excitation filter
- Dichroic mirror
- Emission filter
Assembly Principles
- Cleanliness: Ensure the assembly environment is free of contamination to guarantee ideal optical performance.
- Proper orientation: Each filter should be positioned according to the defined specifications to supply precise light management.
- Secure, gentle mounting: Non-stressful mounting forces should be applied in a controlled manner to ensure stability while protecting components.
- System compatibility: All elements should be verifiably aligned with the system’s optical and mechanical requirements to guarantee reliable, high-quality operation.
Cube Assembly Instructions
Looking for a Different Size?
Chroma recognizes that its standard offerings may not always align with a client’s specific needs. If additional mounting options or measurements are required, Chroma’s team is on hand to assist with all optical filter requirements.

This information has been sourced, reviewed, and adapted from materials provided by Chroma Technology Corp.
For more information on this source, please visit Chroma Technology Corp.