Technical Video, Ltd.

The Light Scrambler was developed to meet the need for a uniform source of the highest luminance (radiance) for use with high loss optical techniques such as polarization, DIC, darkfield and fluorescence microscopy, while pushing the limits of resolution and striving for enhanced sensitivity.

For Video Microscopy/Image Processing:

• Greatly reduces the computer image processing required for background subtraction of non-homogeneous illumination.
• Typically as many as 110/256 grey levels or more may be required; the Light Scrambler can reduce that to 30/256 or better.
• Provides superb images for sub-micron optical sectioning and 3D reconstruction. The better the original image, the better the result.

For Transmitted/DIC:

• Allows the full numerical aperture of the condenser to be used, thus precisely matching the N.A. of the objective, resulting in a vast improvement in lateral resolution and optical sectioning capability. Utilizes the full capabilties of sophisticated optics.

For Epi-Fluorescence:

• The essentially flat illumination allows cells to be selected from a larger area in the field. Depth discrimination is improved. The data baseline obtained is more uniform and repeatable.

For LASERS:

• An efficient and simple way to expand the laser beam and introduce the light into the microscope without complicated alignment problems. Flattens out severe Gaussian distribution.

For All Applications:

• Isolates microscope from mechanical vibration caused by filter wheels and shutters.
• Removes lamp source of RFI & EMI away from the microscope.
• Relocates lamp source of heat away from the microscope and specimens