The fluoroSENS is a versatile bench-top fluorimeter with single-photon counting sensitivity and an excellent water Raman signal-to-noise ratio. It combines measurements of both high-performance steady-state fluorescence spectra and time-resolved phosphorescence decays. The fluoroSENS is a fully integrated, computer-controlled single-photon counting fluorimeter with comprehensive optional accessories.
The light source is a highly stabilized Xenon arc lamp with optical stability better than 0.1% with an auto-start facility in instrument power ON.
The excitation and emission monochromators are designed for high optical throughput and each has a triple grating turret for flexible choice of gratings. This allows the instrument to be optimized for several spectral ranges depending upon customer requirements. Each monochromator is also fitted with continuously variable bilateral knife-edge slits allowing spectral passband from 0.1nm to 10mm.
FluoroSENS is fitted with several key spectroscopy features as standard. These include a reference photodiode detector for corrected excitation spectra, corrected emission channel, integrated order sorting filter wheel and filters as well as an optical attenuator to allow signal optimization.
As standard, the large sample chamber is designed with high light-gathering optics to utilize as much of the sample signal as possible. AS standard the fluoroSENS comes with a single cuvette holder on a positioning stage to ensure sample and signal optimization. Optional samples holders include for example temperature controlled options, four-cell motorized turret, front face sample holder for solid or powder samples, magnetic stirrer, optical fiber adapters, and a motorized 96 well plate reader.
Each fluoroSENS uses a single-photon sensitive photomultiplier detector. This detector can be exchanged for different spectral ranges to ensure complete optimization of customer needs. Optional NIR detectors are also available.
The fluoroSENS uses a new class-leading data acquisition system that provides all signal collection in the one board. This includes a 100M counts per second photon counting module as well as low noise 16-bit analog-to-digital detectors for sample temperature sensing and the reference detectors.
Uniquely, fluoroSENS offers a transmission detector as a sample chamber option to allow the measurement of sample transmission simultaneously with the fluorescence emission or excitation measurements. This allows the user to directly monitor sample photo-bleaching or aggregation etc that might otherwise invalidate the fluorescence measurement.
The system software contains all of the control, data collection, and analysis functions to make high-quality fluorescence measurements. It has the option to save all experimental parameters and to define a quick load measurement function. This function permits routine measurements to be set-up and stored for repeat use. Full flexibility is offered to edit these functions.