Posted in | Raman Microscopes

WITec TrueSurface Microscopy: Topographic Raman Imaging

WITec’s TrueSurface microscopy module introduced topographic Raman imaging to the marketplace. It uses an advanced optical profilometer integrated within the instrument to trace the surface of even roughly-textured or heavily-inclined samples during 3D molecular characterization. This combination of profilometry and Raman spectral acquisition ensures that measurements maintain optimum focus along the surface or at a set distance below while requiring minimal, if any, sample preparation.

As the sensor actively monitors data acquisition with sub-micrometer resolution, its closed-loop operation can compensate for any variations in temperature or humidity during measurements with long integration times, resulting in perfectly sharp and detailed images. Both the profilometry and Raman measurements are acquired through the same optical axis for fast and convenient one-pass operation.

Investigations on pharmaceutical tablet coatings, geological samples, composite emulsions, semiconductors and many other applications can benefit from TrueSurface.

The TrueSurface Principle

Key Features

  • One-pass simultaneous optical profilometry and Raman imaging
  • 3D chemical characterization on coarsely-textured or inclined samples
  • No specialized sample preparation
  • Closed-loop operation eliminates variations during long measurements
  • Data acquisition from a surface, or from a set offset distance 


Topographic Raman microscopy image of micro-structured silicon.

Topographic Raman microscopy image of micro-structured silicon. Image Credit: WITec GmbH

Topographic Raman microscopy image of a pharmaceutical tablet with corresponding spectra.

Topographic Raman microscopy image of a pharmaceutical tablet with corresponding spectra. Image Credit: WITec GmbH

WITecTopographic Raman microscopy image of cellulose fibers. Image Credit: WITec GmbH


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